High-throughput single-molecule fluorescence spectroscopy using parallel detection.

摘要: Solution-based single-molecule fluorescence spectroscopy is a powerful new experimental approach with applications in all fields of natural sciences. The basic concept this technique to excite and collect light from very small volume (typically femtoliter) work concentration regime resulting rare burst-like events corresponding the transit single-molecule. Those are accumulated over time achieve proper statistical accuracy. Therefore advantage extreme sensitivity somewhat counterbalanced by long acquisition time. One way speed up data parallelization. Here we will discuss general address issue, using multispot excitation detection geometry that can accommodate different types novel highly-parallel detector arrays. We illustrate potential correlation (FCS) measurements obtained multipixel single-photon counting detectors.