Substrate-specific clades of active marine methylotrophs associated with a phytoplankton bloom in a temperate coastal environment.

摘要: Marine microorganisms that consume one-carbon (C1) compounds are poorly described, despite their impact on global climate via an influence aquatic and atmospheric chemistry. This study investigated marine bacterial communities involved in the metabolism of C1 compounds. These were relevance to surface seawater chemistry context a bloom was dominated by phytoplankton known produce dimethylsulfoniopropionate. In addition using 16S rRNA gene fingerprinting clone libraries characterize samples taken from transect July 2006, incubated with 13C-labeled methanol, monomethylamine, dimethylamine, methyl bromide, dimethyl sulfide identify microbial populations turnover compounds, DNA stable isotope probing. The [13C]DNA single time point characterized compared denaturing gradient gel electrophoresis (DGGE), fingerprint cluster analysis, library analysis. Bacterial community DGGE fingerprints distinct those obtained nonlabeled suggested some overlap substrate utilization between active methylotroph growing different substrates. Active methylotrophs affiliated Methylophaga spp. several clades undescribed Gammaproteobacteria utilized methylamines (both monomethylamine dimethylamine), sulfide. sequences corresponding assimilating bromide other substrates associated members Alphaproteobacteria (e.g., family Rhodobacteraceae), Cytophaga-Flexibacter-Bacteroides group, unknown taxa. expands diversity provides comprehensive data set for focused cultivation metagenomic analyses future.