Endogenous DHP-sensitive Ca2+channels inPleurodelesoocytes
作者： H. Ouadid , E. Browaeys-Poly , J.P. Vilain , P. Guilbault
DOI: 10.1016/0014-5793(94)00816-7
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摘要: Abstract The double electrode voltage-clamp technique was used to study voltage-dependent Ca 2+ channels in Pleurodeles oocytes. From a holding potential of −80 mV, Ba-current ( I Ba ) (recorded Cl-free solution, = 40 mM) activated at −36.7 ± 4 peaked −11.6 mV and reversed 55 7 n 24). This current slowly (rise time 0.98 0.2 s; 14 −10 mV) not inactivated. Cadmium (Cd , 500 μM) completely inhibited . effect Cd dose-dependent (EC 50 37 5 μM; 5). Moreover, insensitive ω-conotoxin (10 but interestingly this displayed dihydropyridine (DHP) sensitivity. Bay K 8644 (5 μM), DHP activator, increased the peak amplitude manner 5.9 0.6 10) shifted threshold maximum current/voltage relationship towards negative potentials by − 10 mV. Nifedipine antagonist, decreased 80% HP 1.2 6). We concluded that oocytes possess High-Voltage Activated with properties similar L-type channels.
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