Validation of Folate in a Convenient Yeast Assay Suited for Identification of Inhibitors of Alzheimer's Amyloid-β Aggregation

摘要: Previously in the search for chemopreventatives Alzheimer's disease (AD), microbial cells with amyloid-beta (Abeta) protein fusions have been used to screen compounds that reduce aggregation, misfolding or oligomerization of Abeta. In current study, such a system has look at effect folate, whose deficiency associated AD. A folate-deficient yeast strain producing Abeta fused green fluorescent (GFP) spontaneously misfolds/aggregates and suppresses fluorescence was examine effects folinic acid on Abeta-GFP fluorescence. At levels did not affect growth plasmid stability, increasing led fluorescence, suggesting folate can assist preventing Abeta-misfolding/aggregation. This result supports protective role suggests assays may provide significant new approaches testing AD chemopreventatives.