IFN-γ–enhanced allergen penetration across respiratory epithelium augments allergic inflammation

摘要: Background Respiratory allergen contact is the critical event in elicitation and boosting of allergen-specific immune responses, as well induction immediate late inflammatory reactions. Objective We sought to investigate influence various factors allergic inflammation on integrity barrier function respiratory epithelium for allergens. Methods cultured human bronchial epithelial cell line 16HBE14o− a transwell culture system surrogate intact used purified iodine 125–labeled recombinant major birch pollen (rBet v 1) study extent, kinetics, influencing transepithelial penetration. Results Culture supernatants from activated T H 1 clones decreased resistance. A screening (histamine, IFN-γ, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-8, IL-12, TNF-α) identified IFN-γ potent factor capable reducing properties enhancing Increased submucosal concentrations caused by IFN-γ–mediated reduction provoked more than 7-fold augmentation histamine release sensitized basophils. Conclusion These results demonstrate that cell–derived cytokine facilitates penetration through thereby can aggravate inflammation.