[196] Cystathionine synthase (rat liver)

摘要: Publisher Summary This chapter discusses the methods of preparation Cystathionine Synthase (Rat Liver). The radioactive cystathionine formed from homocysteine and serine- 14 C is determined after it separated serine pyruvate on a column cationic ion exchanger, Dowex 50W-X8(H + ). One unit defined as amount enzyme that catalyzes formation 1 micromole per minute. Specific activity expressed units milligram protein. When rat liver homogenate prepared in phosphate buffer described purification procedure, cystathionase well synthase found 105,000 g supernatant. At pH 8.6, however, does not interfere with assay, because there no difference among activities at this presence absence neutralization antibody against obtained rabbit immunized crystalline cystathionase. Crude homogenates also have dehydratase activity, but inhibited by under assay conditions, should usually for synthase.