A cyclophilin gene marker confirming geographical differentiation of Norway spruce populations and indicating viability response on excess soil‐born salinity

摘要: A newly identified cyclophilin-encoding cDNA clone was used to design a codominant inherited EST-PCR marker in Norway spruce. The study of the current minor polymorphism revealed geographically structured differentiation pattern across 17 test populations, showing slight clinal variation south-north through Europe. Based on frequency alleles, isolation-by-distance analysis and Ewens-Watterson test, we conclude that selectively neutral random-drift mutation recently occurred within Alpine population group, thus being responsible for genetic detected. Analysis tolerant susceptible subsets two adjacent Bavarian spruce populations affected by soil-born NaCl pollution, cyclophilin locus also confirms biased genotype frequencies. Considering an unlinked PCR ribosomal protein-encoding EST clone, deviations between pooled sensitive were proven be more significant two-locus homozygous genotypes than each alone. We suggest both loci are linked adaptive genomic regions. Their potential feasibility marker-assisted selection NaCl-tolerant drought resistant tree is discussed.