The multifaceted role of oestrogen in enhancing Chlamydia trachomatis infection in polarized human endometrial epithelial cells

摘要: The oestrogen receptor (ER) α-β+ HEC-1B and the ERα+β+ Ishikawa (IK) cell lines were investigated to dissect effects of exposure on several parameters Chlamydia trachomatis infection. Antibody blockage ERα or ERβ alone simultaneously significantly decreased C. infectivity (45-68%). Addition antagonist, tamoxifen, IK prior after chlamydial infection caused a 30-90% decrease in infectivity, latter due disrupted eukaryotic organelles. In vivo, endometrial glandular epithelial cells are stimulated by hormonally influenced stromal signals. Accordingly, was increased 27% 21% co-cultured with SHT-290 exposed oestrogen. Endometrial cell/epithelial co-culture revealed indirect phosphorylation extracellular signal-regulated kinase calcium-dependant phospholipase A2 production interleukin (IL)-8 IL-6 both uninfected chlamydiae-infected cells. These results indicate that its receptors play multiple roles infection: (i) membrane (mERs) aid entry into host cells, (ii) mER signalling may contribute inclusion development during Additionally, enhancement is affected signals conjunction direct stimulation human epithelia.