Direct molecular identification of Trypanosoma cruzi discrete typing units in domestic and peridomestic Triatoma infestans and Triatoma sordida from the Argentine Chaco.

作者: L. MAFFEY , M. V. CARDINAL , P. C. ORDÓÑEZ-KRASNOWSKI , L. A. LANATI , M. A. LAURICELLA

DOI: 10.1017/S0031182012000856

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摘要: We assessed the distribution of Trypanosoma cruzi Discrete Typing Units (DTUs) in domestic and peridomestic Triatoma infestans sordida specimens collected a well-defined rural area Pampa del Indio, northeastern Argentina. Microscopically-positive bugs were randomly selected with multi-level sampling design, DTUs identified using direct PCR strategies. TcVI predominated 61% 69 T. 56% 9 sordida. TcV was secondary DTU (16%) found 1 specimen (11%). Three (33%) infected TcI, also local Didelphis albiventris opossums. Mixed infections occurred rarely (5%) detected both directly from bugs' rectal ampoule parasite cultures. The bug collection sites significantly associated. Bugs almost exclusively captured domiciles whereas those similarly peridomiciles. All mixed domiciles. TcV-infected fed more often on humans than dogs, TcVI-infected showed reverse pattern. is probable sylvatic vector TcI linked to D. albiventris, could represent domestic/peridomestic cycle.

参考文章(43)
Patricio Diosque, Christian Barnabé, Angel M Padilla, Jorge D Marco, Rubén M Cardozo, Rubén O Cimino, Julio R Nasser, Michel Tibayrenc, Miguel A Basombrı́o, None, Multilocus enzyme electrophoresis analysis of Trypanosoma cruzi isolates from a geographically restricted endemic area for Chagas' disease in Argentina. International Journal for Parasitology. ,vol. 33, pp. 997- 1003 ,(2003) , 10.1016/S0020-7519(03)00139-5
Joel E Cohen, Ricardo E Gurtler, Modeling Household Transmission of American Trypanosomiasis Science. ,vol. 293, pp. 694- 698 ,(2001) , 10.1126/SCIENCE.1060638
Matthew Yeo, Nidia Acosta, Martin Llewellyn, Humberto Sánchez, Susie Adamson, Graham A.J. Miles, Elsa López, Nilsa González, James S. Patterson, Michael W. Gaunt, Antonieta Rojas de Arias, Michael A. Miles, Origins of Chagas disease: Didelphis species are natural hosts of Trypanosoma cruzi I and armadillos hosts of Trypanosoma cruzi II, including hybrids. International Journal for Parasitology. ,vol. 35, pp. 225- 233 ,(2005) , 10.1016/J.IJPARA.2004.10.024
L. Diotaiuti, A. S. Pereira, C. F. Loiola, A. J. Fernandes, J. C. Schofield, J. P. Dujardin, J. C. P. Dias, E. Chiari, Inter-relation of sylvatic and domestic transmission of Trypanosoma cruzi in areas with and without domestic vectorial transmission in Minas Gerais, Brazil Memorias Do Instituto Oswaldo Cruz. ,vol. 90, pp. 443- 448 ,(1995) , 10.1590/S0074-02761995000400002
J.-P. LAURENT, C. BARNABE, V. QUESNEY, S. NOEL, M. TIBAYRENC, Impact of clonal evolution on the biological diversity of Trypanosoma cruzi. Parasitology. ,vol. 114, pp. 213- 218 ,(1997) , 10.1017/S0031182096008414
Enrique E. Montamat, Silvia Arauzo, Juan J. Cazzulo, Elisabet Subías, Characterization by electrophoretic zymograms of 19 Trypanosoma cruzi clones derived from two chronic chagasic patients. Comparative Biochemistry and Physiology B. ,vol. 87, pp. 417- 422 ,(1987) , 10.1016/0305-0491(87)90161-1
Juan M. Burgos, Jaime Altcheh, Margarita Bisio, Tomas Duffy, Helder M.S. Valadares, María Elena Seidenstein, Romina Piccinali, Jorge M. Freitas, Mariano J. Levin, Liliana Macchi, Andrea M. Macedo, Hector Freilij, Alejandro G. Schijman, Direct molecular profiling of minicircle signatures and lineages of Trypanosoma cruzi bloodstream populations causing congenital Chagas disease International Journal for Parasitology. ,vol. 37, pp. 1319- 1327 ,(2007) , 10.1016/J.IJPARA.2007.04.015
Hiroo Higo, Sachio Miura, Masahiro Horio, Tatsuyuki Mimori, Shinjiro Hamano, Takeshi Agatsuma, Tetsuo Yanagi, Arejandro Cruz-Reyes, Norma Uyema, A.Rojas de Arias, Vivian Matta, Hiroshige Akahane, Kenji Hirayama, Tsutomu Takeuchi, Isao Tada, Kunisuke Himeno, Genotypic variation among lineages of Trypanosoma cruzi and its geographic aspects Parasitology International. ,vol. 53, pp. 337- 344 ,(2004) , 10.1016/J.PARINT.2004.06.001
F. Noireau, F. Brenière, J. Ordoñez, L. Cardozo, W. Morochi, T. Gutierrez, M.F. Bosseno, S. Garcia, F. Vargas, N. Yaksic, J.P. Dujardin, C. Peredo, C. Wisnivesky-Colli, Low probability of transmission of Trypanosoma cruzi to humans by domiciliary Triatoma sordida in Bolivia Transactions of The Royal Society of Tropical Medicine and Hygiene. ,vol. 91, pp. 653- 656 ,(1997) , 10.1016/S0035-9203(97)90508-3
A. Riarte, C. Rivas, C. Wisnivesky-Colli, N.J. Schweigmann, A. Alberti, S.M. Pietrokovsky, O. Conti, S. Montoya, Sylvatic American trypanosomiasis in Argentina. Trypanosoma cruzi infection in mammals from the Chaco forest in Santiago del Estero. Transactions of The Royal Society of Tropical Medicine and Hygiene. ,vol. 86, pp. 38- 41 ,(1992) , 10.1016/0035-9203(92)90433-D