Rotavirus A Infections in Community Childhood Diarrhea in the Brazilian Semiarid Region During Postvaccination Era.

摘要: Background Rotavirus A (RVA) is one of the leading causes acute gastroenteritis worldwide; however, few studies assessed RVA genetics with community surveillance. Objectives This study aimed to investigate clinical data, genetic diversity, and coinfection patterns infections in children from 2 36 months old or without childhood diarrhea Brazilian semiarid region during postvaccination era. Methods We enrolled collected socioeconomic/clinical information using a standardized questionnaire fecal samples 291 children. Viral RNA were extracted analyzed quantitative reverse transcription polymerase chain reaction establish diagnosis RVA. Sequencing VP7 VP4 (VP8*) regions phylogenetic analysis performed. Results RVA-negative was associated 24 complete vaccination schedule. Genotype G1P[8] most prevalent (57%), whereas unusual genotypes including G1P[4], G2P[8], G3P[9] also detected. G1- P[8]-positive showed high degrees similarity vaccine strain. coinfections frequently observed, enteroaggregative Escherichia coli copathogen. Conclusions These results demonstrate that genotype and/or VP8* gene segments arising RV1 strain documented these children, suggesting shedding herd vaccination. Moreover, our indicates full important for protection against infections.