Enhancement of Neurospora VS ribozyme cleavage by tuberactinomycin antibiotics.

摘要: Several examples of inhibition the function a ribozyme or RNA-protein complex have shown that certain antibiotics can interact specifically with RNA. There are, however, few positive, rather than negative, effect on an We found micromolar concentrations viomycin, basic, cyclic peptide antibiotic tuberactinomycin group, enhance cleavage derived from Neurospora VS Viomycin decreases by order magnitude concentration magnesium required for cleavage. It also stimulates otherwise insignificant transcleavage reaction enhancing interactions between RNA molecules. The ability viomycin to some RNA-mediated reactions but inhibit others, including translation and Group I intron splicing, demonstrates potential natural selection small molecules during evolution in 'RNA world' may broader implications respect expression activity contemporary cells.