Structure/Function Analysis of the Saccharomyces cerevisiae Trf4/Pol σ DNA Polymerase
作者： David Fitzhugh , Carrie Adams , Zhenghe Wang , Clemence Vu , Michael F. Christman
DOI: 10.1093/GENETICS/160.2.381
关键词:
摘要: The Trf4p/Pol sigma DNA polymerase (formerly kappa) couples replication to the establishment of sister chromatid cohesion. is encoded by two redundant homologs in Saccharomyces cerevisiae, TRF4 and TRF5, that together define a fourth essential nuclear yeast probably all eukaryotes. Here we present thorough genetic analysis founding member this novel family polymerases, TRF4. Analyses mutants carrying 1 34 "surface-targeted" alanine scanning mutations have identified those regions required for Pol sigma's function, its role double-strand break repair, association with chromosomes. data strongly support importance predicted structural similarity beta superfamily as critical repair functions. Surprisingly, five lethal lie outside homology C-terminal region. protein possesses Mg2+-dependent 3' 5' exonuclease activity. Cell cycle reveals associates chromosomes G1, S, G2 phases, but abolished coincident dissolution cohesion at metaphase-to-anaphase transition.
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