In vitro prostanoid release from spinal cord following peripheral inflammation: effects of substance P, NMDA and capsaicin

摘要: 1 Spinal prostanoids are implicated in the development of thermal hyperalgesia after peripheral injury, but specific prostanoid species that involved presently unknown. The current study used an vitro spinal superfusion model to investigate eAect substance P (SP), Nmethyl-d-aspartate (NMDA), and capsaicin on multiple release from dorsal cord naive rats as well underwent injury inflammation (knee joint kaolin/ carrageenan). 2 In rat cords, PGE2 6-keto-PGF1a, not TxB2, levels were increased inclusion SP, NMDA, or perfusion medium. 3 Basal cords animals 5‐72 h elevated relative age-matched cohorts. time course this increase basal coincided with inflammation, assessed by knee circumference. 6-keto-PGF1a injury. 4 From inflammation-evoked levels, SP significantly a dose-dependent fashion. Capsaicin-evoked increases blocked dose-dependently S(+) ibuprofen capsaicin-containing perfusate. 5 These data suggest role for NK-1 receptor activation demonstrate relationship is upregulated response tissue inflammation.