Radioimmunoassays of Prostaglandins and Thromboxanes

作者: Elisabeth Granström

DOI: 10.1007/978-1-4684-2778-3_4

关键词: Prostaglandin biosynthesisBinding siteThromboxanesStandard curveChemistryChromatographyPeripheral plasmaRadioimmunoassay

摘要: Radioimmunoassay is based on a competition between labeled and unlabeled molecules of certain compound for the binding sites an antibody directed against same compound. The presence large amount will lead to extensive displacement from sites, thus, high radioactivity in unbound fraction. absolute amounts assayed are obtained comparison with standard curve known amounts.

参考文章(10)
Bengt Samuelsson, Rodolfo Paoletti, Peter W. Ramwell, Advances in prostaglandin and thromboxane research Raven Press. ,(1980)
Dianna E. Van Orden, Donna B. Farley, Prostaglandin F2α radioimmunoassay utilizing polyethylene glycol separation technique Prostaglandins. ,vol. 4, pp. 215- 233 ,(1973) , 10.1016/0090-6980(73)90040-3
Bernard M. Jaffe, Harold R. Behrman, Charles W. Parker, Radioimmunoassay measurement of prostaglandins E, A, and F in human plasma. Journal of Clinical Investigation. ,vol. 52, pp. 398- 405 ,(1973) , 10.1172/JCI107196
H. Kindahl, L.-E. Edqvist, A. Bane, E. Granstrom, BLOOD LEVELS OF PROGESTERONE AND 15-KETO-13,14-DIHYDRO-PROSTAGLANDIN F2α DURING THE NORMAL OESTROUS CYCLE AND EARLY PREGNANCY IN HEIFERS European Journal of Endocrinology. ,vol. 82, pp. 134- 149 ,(1976) , 10.1530/ACTA.0.0820134
Mats Hamberg, Bengt Samuelsson, Prostaglandin endoperoxides VII. Novel transformations of arachidonic acid in guinea pig lung Biochemical and Biophysical Research Communications. ,vol. 61, pp. 942- 949 ,(1974) , 10.1016/0006-291X(74)90246-0
M. Hamberg, B. Samuelsson, Prostaglandin Endoperoxides. Novel Transformations of Arachidonic Acid in Human Platelets Proceedings of the National Academy of Sciences of the United States of America. ,vol. 71, pp. 3400- 3404 ,(1974) , 10.1073/PNAS.71.9.3400
Elisabeth Granström, Hans Kindahl, Bengt Samuelsson, Radioimmunoassay for Thromboxane B2 Analytical Letters. ,vol. 9, pp. 611- 627 ,(1976) , 10.1080/00032717608059125
Barr Kl, Kirton Kt, Cornette Jc, Forbes Ad, Radioimmunoassay of prostaglandins. Journal of Reproductive Medicine. ,vol. 9, pp. 355- ,(1972)
Speroff L, Anderson Gg, Hobbins Jc, Gordon Jw, Auletta Fj, Brock Wa, Caldwell Bv, Development and application of a radioimmunoassay for F prostaglandins. Journal of Reproductive Medicine. ,vol. 9, pp. 361- 371 ,(1972)