Differential cholinoceptor subtype-dependent activation of signal transduction pathways in neonatal versus adult rat atria
作者： Enri S. Borda , Claudia Perez Leiros , Juan Jose Camusso , Sandra Bacman , Leonor Sterin-Borda
DOI: 10.1016/S0006-2952(96)00866-0
关键词:
摘要: In this study, we investigated the expression and distribution of muscarinic cholinergic receptors (mAChRs) different signaling pathways associated with mAChR activation in atria isolated from adult neonatal rats. Carbachol stimulation mAChRs both rat led to a negative inotropic response phosphoinositide hydrolysis, an increase cyclic GMP levels, decrease AMP production. However, compared atria, showed hypersensitivity contractile effect induced by carbachol. Pharmacological analysis antagonists indicated that M1 M2 subtypes are important mediators carbachol atria. contrast, agonist was coupled only subtype. Moreover, increased number total labeled atrial membranes those adults. Although predominant population is expressed at stages development studied, competition binding parameters calculated for presence high-affinity sites, higher affinity neonates than These results suggest differences observed between their may be related differential and/or changes functional coupling during development.
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sci-hub.se 参考文章(37)
Van Rossum Jm, Cumulative dose-response curves. II. Technique for the making of dose-response curves in isolated organs and the evaluation of drug parameters. Archives internationales de pharmacodynamie et de thérapie. ,vol. 143, pp. 299- 330 ,(1963)
B L Brown, J D M Albano, R P Ekins, A M Sgherzi, W Tampion, A simple and sensitive saturation assay method for the measurement of adenosine 3':5'-cyclic monophosphate. Biochemical Journal. ,vol. 121, pp. 561- 562 ,(1971) , 10.1042/BJ1210561
J. Lechleiter, R. Hellmiss, K. Duerson, D. Ennulat, N. David, D. Clapham, E. Peralta, Distinct sequence elements control the specificity of G protein activation by muscarinic acetylcholine receptor subtypes. The EMBO Journal. ,vol. 9, pp. 4381- 4390 ,(1990) , 10.1002/J.1460-2075.1990.TB07888.X
I Coupry, E Duzic, S.M. Lanier, Factors determining the specificity of signal transduction by guanine nucleotide-binding protein-coupled receptors. II. Preferential coupling of the alpha 2C-adrenergic receptor to the guanine nucleotide-binding protein, Go. Journal of Biological Chemistry. ,vol. 267, pp. 9852- 9857 ,(1992) , 10.1016/S0021-9258(19)50172-7
J W Fleming, P L Wisler, A M Watanabe, Signal transduction by G proteins in cardiac tissues Circulation. ,vol. 85, pp. 420- 433 ,(1992) , 10.1161/01.CIR.85.2.420
Sandra Bagman, Leonor Sterin Borda, Juan Jose Camusso, Osvaldo Hubscher, Roberto Arana, Enri S. Borda, Circulating antibodies against neurotransmitter receptor activities in children with congenital heart block and their mothers. The FASEB Journal. ,vol. 8, pp. 1170- 1176 ,(1994) , 10.1096/FASEBJ.8.14.7958624
E. G. Peralta, A. Ashkenazi, J. W. Winslow, D. H. Smith, J. Ramachandran, D. J. Capon, Distinct primary structures, ligand-binding properties and tissue-specific expression of four human muscarinic acetylcholine receptors. The EMBO Journal. ,vol. 6, pp. 3923- 3929 ,(1987) , 10.1002/J.1460-2075.1987.TB02733.X
M. Marlene Hosey, Diversity of structure, signaling and regulation within the family of muscarinic cholinergic receptors. The FASEB Journal. ,vol. 6, pp. 845- 852 ,(1992) , 10.1096/FASEBJ.6.3.1740234
H I Yamamura, M Watson, W R Roeske, R A Evans, Differential ontogeny of putative M1 and M2 muscarinic receptor binding sites in the murine cerebral cortex and heart. Journal of Pharmacology and Experimental Therapeutics. ,vol. 235, pp. 612- 618 ,(1985)
J H Brown, S L Brown, Agonists differentiate muscarinic receptors that inhibit cyclic AMP formation from those that stimulate phosphoinositide metabolism. Journal of Biological Chemistry. ,vol. 259, pp. 3777- 3781 ,(1984) , 10.1016/S0021-9258(17)43162-0