[23] Basal and activated in vitro transcription in plants by RNA polymerase II and III
作者： Hao Fan , Masahiro Sugiura
DOI: 10.1016/S0076-6879(96)73025-2
关键词:
摘要: Publisher Summary This chapter describes the procedures for preparation of basal and activated in vitro transcription systems RNA polymerase II (Pol II)-dependent genes. The system also supports I- III-dependent transcription. Development plant is critical elucidating biochemical mechanism gene its regulation plants. Tobacco nongreen cultured BY-2 cells are a good source this purpose because cell line has fastest growth rate among lines reported tobacco provides powerful tool with which to analyze vivo by well-established transgenic technique. As do not differentiate response light or any other stimuli, they give that lacks tissue-specific light-responsive factors. By combination active fractions from organs tissues genes question specifically transcribed, cis-elements trans-factors involved their expected be detected .
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