Assembly and disassembly of the Drosophila RNA polymerase II complex during transcription.
作者： J T Kadonaga
DOI: 10.1016/S0021-9258(19)39847-3
关键词:
摘要: Initiation of mRNA synthesis by RNA polymerase II occurs a multistep pathway that involves the along with several auxiliary factors. Here I describe an analysis kinetics and mechanism action Drosophila using detergent Sarkosyl to inhibit different steps in leading transcription initiation. The template DNA factors assemble into partially Sarkosyl-resistant initiation complex kinetically first order time course characterized t1/2 about 3 min, subsequent conversion elongating rapidly (less than 5 s). During formation two phosphodiester bonds, there is stepwise transition from 0.25% polymerase. In contrast mammalian systems, extracts possess ability carry out many rounds (approximately 10) vitro. Finally, are committed as complex, but then no longer appear remain after transcription. This finding suggests complete assembly disassembly during each round II.
sci-hub.st 参考文章(29)
M Sawadogo, R G Roeder, Energy requirement for specific transcription initiation by the human RNA polymerase II system. Journal of Biological Chemistry. ,vol. 259, pp. 5321- 5326 ,(1984) , 10.1016/S0021-9258(17)42993-0
J A Arias, W S Dynan, Promoter-dependent Transcription by RNA Polymerase II Using Immobilized Enzyme Complexes Journal of Biological Chemistry. ,vol. 264, pp. 3223- 3229 ,(1989) , 10.1016/S0021-9258(18)94055-X
R C Conaway, J W Conaway, ATP activates transcription initiation from promoters by RNA polymerase II in a reversible step prior to RNA synthesis. Journal of Biological Chemistry. ,vol. 263, pp. 2962- 2968 ,(1988) , 10.1016/S0021-9258(18)69162-8
Alan G. Saltzman, Roberto Weinmann, Promoter specificity and modulation of RNA polymerase II transcription. The FASEB Journal. ,vol. 3, pp. 1723- 1733 ,(1989) , 10.1096/FASEBJ.3.6.2649403
D K Hawley, R G Roeder, Separation and partial characterization of three functional steps in transcription initiation by human RNA polymerase II. Journal of Biological Chemistry. ,vol. 260, pp. 8163- 8172 ,(1985) , 10.1016/S0021-9258(17)39577-7
D.K. Hawley, R.G. Roeder, Functional Steps in Transcription Initiation and Reinitiation from the Major Late Promoter in a HeLa Nuclear Extract Journal of Biological Chemistry. ,vol. 262, pp. 3452- 3461 ,(1987) , 10.1016/S0021-9258(18)61372-9
D. Reinberg, R.G. Roeder, Factors involved in specific transcription by mammalian RNA polymerase II. Purification and functional analysis of initiation factors IIB and IIE. Journal of Biological Chemistry. ,vol. 262, pp. 3310- 3321 ,(1987) , 10.1016/S0021-9258(18)61505-4
A Fire, M Samuels, P A Sharp, Interactions between RNA polymerase II, factors, and template leading to accurate transcription. Journal of Biological Chemistry. ,vol. 259, pp. 2509- 2516 ,(1984) , 10.1016/S0021-9258(17)43382-5
M Samuels, A Fire, P A Sharp, Dinucleotide priming of transcription mediated by RNA polymerase II. Journal of Biological Chemistry. ,vol. 259, pp. 2517- 2525 ,(1984) , 10.1016/S0021-9258(17)43383-7
Patricio Gariglio, Janice Buss, Melvin H. Green, Sarkosyl activation of rna polymerase activity in mitotic mouse cells. FEBS Letters. ,vol. 44, pp. 330- 333 ,(1974) , 10.1016/0014-5793(74)81170-1