Identification, Stereoconfiguration, Chromatographic and Fluorescence Properties of Natural Pterins

摘要: Several years ago, we reported the identification of dictyopterin as 6-[D-threo]-1’, 2’-dihydroxy propyl-pterin1. Such a result had been obtained after numerous and tedious purification steps. In order to facilitate determination stereoconfiguration natural pterins, which are found in very small amounts living organisms, aimed find sensitive method. This was achieved using chiral HPLC. The separation D-and L-enantiomers 6-(polyhydroxypropyl)-pterins by ligand exchange chromatography reversed-phase column with mobile phase containing D-phenylalanine modifier Cu(II) metal ion. enantiomeric allowed some pterins be deterriiined picomole range, comparison their chromatographic fluorescence properties those reference pterins2. present paper briefly reports an aspect method dealing quantum yields describes one application answers following questions : what is urinary monapterin ? Are there any differences human urine from cancer non-cancer patients?