Assessment of cytotoxic and genotoxic potential of pyracarbolid by Allium test and micronucleus assay.
作者： Arzu Özkara , Dilek Akyıl , Yasin Eren , S Feyza Erdoğmuş , Muhsin Konuk
DOI: 10.3109/01480545.2014.966831
关键词:
摘要: AbstractThe present study evaluates the cytotoxic and genotoxic potential of pyracarbolid using both micronuleus (MN) assay, in human lymphocytes, Allium cepa root meristem cells. In test, EC50 value was determined order to selecting test concentrations for assay tips were treated with 25 ppm (EC50/2), 50 ppm (EC50) 100 ppm (EC50 × 2) pyracarbolid. One percent dimethyl sulphoxide (DMSO) methyl methane sulfonate (MMS) used as negative positive controls, respectively. micronucleus cultures four (250, 500, 750 1000 µg/ml) 24 48 h, controls also experiment parallely. The results showed that mitotic index (MI) significantly reduced increasing concentration at each exposure time. It obtained prophase metaphase decreased all ex...
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