Impact of consumption of different levels of Bifidobacterium lactis hn019 on the intestinal microflora of elderly human subjects

摘要: BACKGROUND: Age-related changes in the physiology and intestinal function of elderly render them more susceptible to gut-related illnesses. Probiotic dietary supplementation has been shown enhance health indices elderly. OBJECTIVE: To determine effect three different doses [5 x 109 CFU/day (high), 1.0 (medium) 6.5 107 (low)] Bifidobacterium lactis HN019 (DR10TM) on flora human subjects dose response effect. DESIGN: Randomised, double-blind placebo-controlled intervention study consisting four groups 20 (over 60 years old) volunteers. Each volunteer consumed 250 mL per day reconstituted skim milk (RSM) which either did not contain any probiotic supplement (placebo group) or contained B. at levels (low, medium high groups). The comprised stages: a 2-week pre-intervention (without supplement), followed by 4 weeks test feeding (dietary intervention) then washout period. RESULTS: After intervention, statistically significant increases bifidobacteria, lactobacilli enterococci were observed. At end 4-week period mean number bifidobacteria recorded placebo group 9.31 +/- 0.01 log CFU/g faeces. In high, low significantly (p < 0.006) higher (9.88 0.1, 9.75 0.14 9.74 0.11 faeces, respectively), when compared respective levels. There no differences superior 0.05) between responses groups, indicating that even lowest tested augmented bifidobacteria. Similar trends observed for enterococci. contrast, counts enterobacteria reduced all groups. CONCLUSION: present showed with increased resident counts. addition, also increased. Based this already published clinical evidence (4, 5, 8, 9) we conclude that, is suitable (6.5 CFU/day) able confer desired microflora.