Effect of heparin on the production of matrix metalloproteinases and tissue inhibitors of metalloproteinases by human dermal fibroblasts

摘要: The influence of heparin and a fragment devoid anticoagulant activity on the production matrix metalloproteinases tissue inhibitors by human dermal fibroblasts was studied. Doses (0.1-400 microg/ml) responses were performed data obtained similar whatever or used. basal expression collagenase decreased quasi-linearly with increasing doses heparins from 1 to 400 microg/ml. TIMP-1 levels not affected supplementing serum free culture medium heparins. On contrary, at low concentration, i.e. 1-10 microg/ml, stimulated secretion both 72-kDa gelatinase (1.4-1.6-fold) particularly TIMP-2 (>4-fold). At high doses, MMP-2 returned levels. These results suggested that local concentration released mast cells could be instrumental in modulating fibroblast growth proteolytic phenotype.