Cloning of the human homologue of the murine flt3 ligand : a growth factor for early hematopoietic progenitor cells

摘要: Using a fragment of the murine flt3 ligand as probe, we have succeeded in cloning human from T-cell lambda gt10 cDNA library. The and ligands are 72% identical at amino acid level. Analysis multiple clones shows that alternative splicing mRNA can occur number positions. A recombinant soluble form stimulates proliferation colony formation subpopulation bone marrow cells CD34+ enriched for primitive hematopoietic cells. In addition, also expressing receptors. Northern blot analysis widespread expression transcripts tissues.