Efficacy of a novel bacteriocin isolated from Lysinibacillus sp. against Bacillus pumilus
作者： Varish Ahmad , Khurshid Ahmad , Mohammad Hassan Baig , Hind A AL-Shwaiman , Manal M Al Khulaifi
DOI: 10.1016/J.LWT.2018.12.021
关键词:
摘要: Abstract A strain of Lysinibacillus, isolated from spoiled fruits and vegetable wastes, was found having an inhibitory effect against foodborne pathogens. Subjected to study different physico-chemical parameters followed by characterization based on the 16S rRNA gene, it exhibiting resemblance L. boronitolerans (99%), xylanilyticus (98%) macroides (97%) respectively. The isolate annotated as Lysinibacillus JX402121 that production capacity specific product recognized bacteriocin during growth in medium further purified homogeneity ammonium sulphate precipitation gel filtration chromatography. MALDI-TOF/MS analysis substance revealed be a 51 kDa bacteriocin, showing greater with catalytic bacterial protein, uracil-5-methyl transferase. Based characteristics N-terminal (IKKAPVQKNENYEV) sequence, this novel representing class III bacteriocin. This highly active pathogens, Bacillus pumilus (MIC-22 μg/mL), which is responsible for pumilacidins like food toxin. Having great potential will utilized acts bio-preservative agent food-borne
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