Differential involvement of Gα12 and Gα13 in receptor-mediated stress fiber formation.
作者： Antje Gohla , Stefan Offermanns , Thomas M. Wilkie , Günter Schultz
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摘要: The ubiquitously expressed heterotrimeric guanine nucleotide-binding proteins (G-proteins) G12 and G13 have been shown to activate the small GTPase Rho. Rho stimulation leads a rapid remodeling of actin cytoskeleton subsequent stress fiber formation. We investigated involvement or in formation induced through variety Gq/G11-coupled receptors. Using fibroblast cell lines derived from wild-type Gαq/Gα11-deficient mice, we show that agonist-dependent activation endogenous receptors for thrombin lysophosphatidic acid heterologously bradykinin B2, vasopressin V1A, endothelin ETA, serotonin 5-HT2C either presence absence Gαq/Gα11. Stress assembly muscarinic M1 metabotropic glutamate subtype 1α was dependent on Gq/G11proteins. ETB angiotensin AT1A failed induce Lysophosphatidic acid, receptor-mediated Gα13 involved epidermal growth factor (EGF) receptors, whereas thrombin, V1A accumulation via Gα12 an EGF receptor-independent manner. Our data demonstrate many Gαq Gα11 this involves Gα12or Gα13/EGF pathway.
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