Die Oberfläche des Endokards des Rattenherzens. Rasterelektronenmikroskopische Untersuchungen

摘要: Abstract Introduction : The difficulties in the observation of inner surface blood vessels and heart mainly arise from known preparation techniques („Hautchen“, replicas, light microscopy, freeze etching). Scanning electron microscopy may overcome some these because its great depth focus possibility to study large areas. Material Methods: 12 adult male Wistar rats were fixed by vivo perfusion via abdominal aorta with 3% glutaraldehyde + saccharose +3% dextran (15–20,000 m.w.) phosphate buffer (0.1 m, pH 7.2) under ether anesthesia. pressure was 100–120 mm Hg. Tissue dehydration performed through a graded alcohol series followed drying vacuum chamber. After this hearts dissected specimens coated 100 A carbon 300 gold. They observed JSM-U3 (Jeol) Stereoscan (Cambridge). number endothelial cell nuclei middle part septum (fig. 1–3) latero-anterior wall left ventricle 4), lateral right ventricle, atrium, tricuspid, mitral, aortic 10–12) valves have been determined relation standard sur/face area 10,000 μ 2 . results examined multiple comparison test Scheffe (see table). It is suggested that more active or passive motility structures denser population their cells. Results Discussion: In areas borders are seen as network slightly elevated rims which correspond microscopic lines after silver impregnation vessel endothelium 4). Some cells, especially on valves, possess microvilli wart-like processes 6, 11). pseudopode-like protrusions cytoplasm, too 12). relief subendothelial connective tissue fibers myocardial can be detected thin cytoplasm cells 2, 3, 7, 9). exhibit cross-banding if very 3). devided into (less than 0.5 diameter; fig. 3) thick (1–1.5 9) probably solitary reticulum collagen respectively bundles them. fiber direction varies stream different localizations Thus, not only tension forces but also other factors influence position endocardial fibers. bands consisting crossly arranged rolls effect shrunk elastic