Frozen-fractured images of blood capillaries in heart tissue.

作者: Lee V. Leak

DOI: 10.1016/S0022-5320(71)80147-8

关键词: EndotheliumMembraneConnective tissueBiologyCapillary actionVesicleFixation (histology)Biomedical engineeringElectron microscopePathologyUltrastructure

摘要: The ultrastructure of the mammalian heart capillary has been studied with technique freeze-etching which lends itself as a very useful method for studies designed to investigate specialized surface features endothelial plasma membrane. Plasmalemmal invaginations have shown populate membrane by many investigators using conventional procedures electron microscopy (chemical fixation and plastic embedding). freezeetching, however, allows more critical evaluation organization frequency micropinocytotic vesicles over both connective tissue luminal surfaces endothelium especially in replicas present large areas en face views. varies considerably from one square micron another. However, when are taken into consideration distribution front similar. This observation is discussed light current morphological tracer preparatory microscopy. three-dimensional relationship various tunics comprising blood its adjoining muscle fibers also considered.

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