Lactobacilli as effectors of host functions: no influence on the activities of enzymes in enterocytes of mice.

摘要: Preparations of lactobacilli are often used as dietary supplements to improve the growth and efficiency in utilizing food animals commercial value. We tested an experimental model whether effects on utilization by may be due alteration activities absorptive enzymes small bowel. Germfree mice housed isolators under tightly controlled conditions were monoassociated with one four strains indigenous Lactobacillus spp. From 1 5 weeks later, activity alkaline phosphatase was assayed homogenates segments upper intestines associated matched germfree controls. The specific enzyme same two groups. In other experiments, epithelial cells isolated from eight (octa-associated) those for phosphatase, phosphodiesterase, thymidine kinase activities. harvested sequentially tips villi toward crypts Lieberkuhn intestines. all preparations, both types yielded equivalent mass (wet weight) cells. protein content reflected mass. microvillous membrane phosphodiesterase cytosol or not contained bacteria. Therefore, any animal observed when a direct bacteria host animal.(ABSTRACT TRUNCATED AT 250 WORDS) Images