Intron conservation across the prokaryote-eukaryote boundary: structure of the nuclear gene for chloroplast glyceraldehyde-3-phosphate dehydrogenase from maize.

摘要: Abstract The nuclear gene encoding chloroplast glyceraldehyde-3-phosphate dehydrogenase (GAPDH) from maize has been cloned and sequenced. The is G + C rich in its coding sequences and, addition, contains a CpG-rich region surrounding the promoter. Further upstream several enhancer-like repetitions have identified that may control light- phytochrome-mediated expression of this gene. interrupted by three introns. Introns 1 2 are located within sequence transit peptide, dividing it into parts, each containing one major homology blocks typical for peptides nucleus-encoded proteins. Intron 3 at codon 166 (glycine) same nucleotide position as intron GAPDH nematode Caenorhabditis elegans, suggesting was present parental which these two modern descendants originated. divides protein constituent domains, NAD-binding catalytic domain, immediately after helix alpha homologous to 9 alcohol dehydrogenase, thereby confirming prediction Branden et al. on basis gene-protein structure correlations placement introns [Branden, C.I., Eklund, H., Cambillau, C. & Pryor, A.J. (1984) EMBO J. 3, 1307-1310]. These results suggest an archetypical relic early evolution, whereas were implicated evolution peptides.