A 1.5-megabase yeast artificial chromosome contig from human chromosome 10q11.2 connecting three genetic loci (RET, D10S94, and D10S102) closely linked to the MEN2A locus.

摘要: Abstract The genetic loci RET, D10S94, and D10S102 from human chromosome 10q11.2 are very closely linked to a locus responsible for the multiple endocrine neoplasia type 2 (MEN2A MEN2B) medullary thyroid carcinoma (MTC1) familial cancer syndromes. We have constructed 1.5-megabase contig consisting of six genomic yeast artificial clones which include these define their physical order. A critical crossover event has been identified within map interval; this places MEN2A centromeric defines orientation on chromosome. The order markers centromere-RET-D10S94-D10S102-telomere. In addition, microsatellite repeat polymorphism with heterozygosity 71% at RET restriction fragment length 42% detected by lambda clone D10S94 developed high-resolution linkage mapping predictive diagnostic testing. These data place three important contiguous map, narrow MEN2 disease interval, provide framework further candidate gene identification efforts. Placement along clone-based continued expansion will also facilitate efforts determine relationship distance near centromeres chromosomes.