Characterization of K562 cells following introduction of a mutant N-ras gene.
作者： Jacqueline T. Thorn , Alison V. Todd , Genevieve M. Croaker , Harry J. Iland
DOI: 10.1016/0145-2126(93)90137-A
关键词:
摘要: A mutant human N-ras gene (codon 61, C to substitution) was electroporated into the leukemic cell line K562, originally derived from a patient with chronic myeloid leukemia (CML) in blast crisis. Despite confirmation of integration and expression, transfected cells exhibited no growth advantage when characterized suspension cultures clonogenic assays, serum deprivation impaired proliferation both normal equally. subclone containing displayed rate differentiation potential identical that non-transfected cells. The failure mutations modify K562 behavior is keeping infrequent observation blastic transformation CML.
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