Avoiding common numts to provide reliable species identification for tiger parts

作者: Kelly I. Morgan , Kyle M. Ewart , Truong Q. Nguyen , Frankie T. Sitam , Kanita Ouitavon

DOI: 10.1016/J.FSIR.2020.100166

关键词:

摘要: Abstract Tigers are killed to supply a demand for many wildlife products despite ban on commercial international trade. As populations decrease, from substitute species (i.e. lions and leopards) have been fraudulently sold as tiger. DNA forensic techniques needed definitively identify tiger in order secure prosecutions although this is complicated by the presence of numts. Therefore, we developed validated CO1 genetic marker that preferentially amplifies mtDNA region excludes nuclear pseudogene, which expect be use casework.

参考文章(36)
Species identification using DNA loci Linacre, A. and Tobe, S.S. <https://researchrepository.murdoch.edu.au/view/author/Tobe, Shanan.html>ORCID: 0000-0002-4854-6278 <http://orcid.org/0000-0002-4854-6278> (2009) Species Identification Using DNA Loci. In: Linacre, A., (ed.) Forensic Science in Wildlife Investigations. CRC Press, pp. 61-94.. pp. 61- 94 ,(2009) , 10.1201/9780849304118-9
Heidy Sierra, Miguel Cordova, Chih-Shan Jason Chen, Milind Rajadhyaksha, Confocal Imaging–Guided Laser Ablation of Basal Cell Carcinomas: An Ex Vivo Study Journal of Investigative Dermatology. ,vol. 135, pp. 612- 615 ,(2015) , 10.1038/JID.2014.371
De-Xing Zhang, Godfrey M. Hewitt, Nuclear integrations: challenges for mitochondrial DNA markers Trends in Ecology and Evolution. ,vol. 11, pp. 247- 251 ,(1996) , 10.1016/0169-5347(96)10031-8
Junghwa An, Mu-yeong Lee, Mi-Sook Min, Mun-Han Lee, Hang Lee, A molecular genetic approach for species identification of mammals and sex determination of birds in a forensic case of poaching from South Korea Forensic Science International. ,vol. 167, pp. 59- 61 ,(2007) , 10.1016/J.FORSCIINT.2005.12.031
L.M.I. Webster, R. McEwing, Resurrecting the Dodo: Positive control DNA for species identification Forensic Science International: Genetics Supplement Series. ,vol. 4, ,(2013) , 10.1016/J.FSIGSS.2013.10.071
Qiu-Hong Wan, Sheng-Guo Fang, Application of species-specific polymerase chain reaction in the forensic identification of tiger species Forensic Science International. ,vol. 131, pp. 75- 78 ,(2003) , 10.1016/S0379-0738(02)00398-5
Shanan S. Tobe, Adrian Linacre, DNA typing in wildlife crime: recent developments in species identification Forensic Science Medicine and Pathology. ,vol. 6, pp. 195- 206 ,(2010) , 10.1007/S12024-010-9168-7
H. Song, J. E. Buhay, M. F. Whiting, K. A. Crandall, Many species in one: DNA barcoding overestimates the number of species when nuclear mitochondrial pseudogenes are coamplified Proceedings of the National Academy of Sciences of the United States of America. ,vol. 105, pp. 13486- 13491 ,(2008) , 10.1073/PNAS.0803076105
Sunil Kumar Verma, Kasturi Prasad, Narayan Nagesh, Mehar Sultana, Lalji Singh, Was elusive carnivore a panther? DNA typing of faeces reveals the mystery Forensic Science International. ,vol. 137, pp. 16- 20 ,(2003) , 10.1016/S0379-0738(03)00277-9