Clinical chorioamnionitis at term II: the intra-amniotic inflammatory response

作者: Roberto Romero , Piya Chaemsaithong , Steven J. Korzeniewski , Adi L. Tarca , Gaurav Bhatti

DOI: 10.1515/JPM-2015-0045

关键词:

摘要: Objective: Recent studies indicate that clinical chorioamnionitis is a heterogeneous condition and only approximately one-half of the patients have bacteria in amniotic cavity, which often associated with intra-amniotic inflammation. The objective this study to characterize nature inflammatory response within cavity at term according presence or absence 1) 2) Materials methods: A retrospective cross-sectional case-control was conducted examine cytokine chemokine concentrations fluid (AF). Cases consisted women (n=45). Controls were uncomplicated pregnancies who did not inflammation labor (n=24). Women classified results AF cultures, broad-range polymerase chain reaction coupled electrospray ionization mass spectrometry, concentration interleukin-6 (IL-6) into those: without inflammation, microbial-associated 3) detectable bacteria. 29 cytokines/chemokines determined using sensitive specific V-PLEX immunoassays. Results: pro- anti-inflammatory such as interferon gamma (IFN-γ), tumor necrosis factor alpha (TNF-α), interleukin-4 (IL-4), macrophage protein-1 beta (MIP-1β), interleukin-8 (IL-8) (except Eotaxin-3) significantly higher than controls (term inflammation); those bacteria, had dramatic differential expression cytokines chemokines compared spontaneous However, no difference could be detected pattern between bacteria; but behavior similar term. Conclusions: Patients upregulation assessed by cytokines. subset does infection/inflammation, demonstrated elevated inflammation-related proteins, when pregnancies, suggesting over-diagnosis. These observations constitute first characterization cytokine/chemokine network

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