MicroRNA-21 protects against the H2O2-induced injury on cardiac myocytes via its target gene PDCD4

摘要: Reactive oxygen species (ROS)-induced cardiac cell injury via expression changes of multiple genes plays a critical role in the pathogenesis numerous heart diseases. MicroRNAs (miRNAs) comprise novel class endogenous, small, noncoding RNAs that negatively regulate about 30% degradation or translational inhibition their target mRNAs. Currently, effects ROS on miRNA and roles miRNAs ROS-mediated myocytes are uncertain. Using quantitative real-time RT-PCR (qRT-PCR), we demonstrated microRNA-21 (miR-21) was upregulated after treatment with hydrogen peroxide (H2O2). To determine potential H2O2-mediated gene regulation cellular injury, miR-21 downregulated by inhibitor pre-miR-21. H2O2-induced death apoptosis were increased decreased Programmed 4 (PDCD4) regulated direct myocytes. Pre-miR-21-mediated protective effect myocyte inhibited H2O2-treated cells adenovirus-mediated overexpression PDCD4 without binding site. Moreover, Activator protein 1 (AP-1) downstream signaling molecule involved miR-21-mediated The results suggest is sensitive to H2O2 stimulation. participates functional modulation might play an essential diseases related such as hypertrophy, failure, myocardial infarction, ischemia/reperfusion injury.