Platelet-like particle formation in the human megakaryoblastic leukaemia cell lines, MEG-01 and MEG-01s

摘要: The platelet-sized particle formation in the human megakaryoblastic leukaemia cell line MEG-01 and its subline MEG-01s was examined. cells spontaneously released particles into culture medium, which occasionally extended cytoplasmic processes similar to those of megakaryocyte proplatelets. Scanning electron microscopic images showed elongated from blebs on surface were constricted between segments platelet size. Immunofluorescence staining with anti-tubulin antibody that contained microtubules organized a ring, is characteristic circulating platelets. Some particles, probably by ruptures at sites process constriction, metabolically active an MTT assay (about 50%). also expressed platelet-specific glycoproteins GPIIb, IIIa GMP-140. Rarely, response thrombin, underwent shape change spherical irregular membrane protrusions fine filopodia, aggregating one another. had increased GMP-140 (P-selectin) expression addition thrombin. These results show usefulness lines for study thrombopoiesis.