New Interface for Purification of Proteins: One-Dimensional TiO2 Nanotubes Decorated by Fe3O4 Nanoparticles
作者： Rudolf Kupcik , Pavel Rehulka , Zuzana Bilkova , Hanna Sopha , Jan M. Macak
关键词:
摘要: In this work, a high surface area interface, based on anodic one-dimensional (1D) TiO2 nanotubes homogeneously decorated by Fe3O4 nanoparticles (TiO2NTs@Fe3O4NPs) is reported for the first time an unprecedented purification of His-tagged recombinant proteins. Excellent results were achieved from model protein mixture, as well whole cell lysate (with ubiquitin). Compared to conventional immobilized-metal affinity chromatography (IMAC) system, specific isolation selected proteins behalf other was significantly enhanced TiO2NTs@Fe3O4NPs interface under optimized binding and elution conditions. The combination properties, magnetic features, biocompatibility, ease preparation material consisting two basic metal oxides makes it suitable candidate future in biotechnology. principally new bears large potential open pathways disco...
acs.org
sci-hub.se 参考文章(58)
Gülfem Aygar, Murat Kaya, Necati Özkan, Semra Kocabıyık, Mürvet Volkan, Preparation of silica coated cobalt ferrite magnetic nanoparticles for the purification of histidine-tagged proteins Journal of Physics and Chemistry of Solids. ,vol. 87, pp. 64- 71 ,(2015) , 10.1016/J.JPCS.2015.08.005
Joanne Crowe, Heinz Döbeli, Reiner Gentz, Erich Hochuli, Dietrich Stüber, Karsten Henco, 6xHis-Ni-NTA chromatography as a superior technique in recombinant protein expression/purification. Methods of Molecular Biology. ,vol. 31, pp. 371- 387 ,(1994) , 10.1385/0-89603-258-2:371
Hamed Arami, Amit Khandhar, Denny Liggitt, Kannan M. Krishnan, In vivo delivery, pharmacokinetics, biodistribution and toxicity of iron oxide nanoparticles Chemical Society Reviews. ,vol. 44, pp. 8576- 8607 ,(2015) , 10.1039/C5CS00541H
M. Bloemen, L. Vanpraet, M. Ceulemans, T. N. Parac-Vogt, K. Clays, N. Geukens, A. Gils, T. Verbiest, Selective protein purification by PEG–IDA-functionalized iron oxide nanoparticles RSC Advances. ,vol. 5, pp. 66549- 66553 ,(2015) , 10.1039/C5RA11614G
Qian Ni, Bing Chen, Shaohua Dong, Lei Tian, Quan Bai, Preparation of core-shell structure Fe3 O4 @SiO2 superparamagnetic microspheres immoblized with iminodiacetic acid as immobilized metal ion affinity adsorbents for His-tag protein purification. Biomedical Chromatography. ,vol. 30, pp. 566- 573 ,(2016) , 10.1002/BMC.3584
M.-L. Michel, P. Tiollais, Hepatitis B vaccines: protective efficacy and therapeutic potential. Pathologie Biologie. ,vol. 58, pp. 288- 295 ,(2010) , 10.1016/J.PATBIO.2010.01.006
Gary Walsh, Biopharmaceutical benchmarks 2010 Nature Biotechnology. ,vol. 28, pp. 917- 924 ,(2010) , 10.1038/NBT0910-917
Thomas J Webster, Enhanced osteoblast adhesion to drug-coated anodized nanotubular titanium surfaces. International Journal of Nanomedicine. ,vol. 3, pp. 257- 264 ,(2008) , 10.2147/IJN.S2552
Hiroaki Tsuchiya, Jan M. Macak, Lenka Müller, Julia Kunze, Frank Müller, Peter Greil, Sannakaisa Virtanen, Patrik Schmuki, Hydroxyapatite growth on anodic TiO2 nanotubes Journal of Biomedical Materials Research Part A. ,vol. 77, pp. 534- 541 ,(2006) , 10.1002/JBM.A.30677
Randy Chi Fai Cheung, Jack Ho Wong, Tzi Bun Ng, Immobilized metal ion affinity chromatography: a review on its applications Applied Microbiology and Biotechnology. ,vol. 96, pp. 1411- 1420 ,(2012) , 10.1007/S00253-012-4507-0