MiR-423-5p Regulates Cells Apoptosis and Extracellular Matrix Degradation via Nucleotide-Binding, Leucine-Rich Repeat Containing X1 (NLRX1) in Interleukin 1 beta (IL-1β)-Induced Human Nucleus Pulposus Cells

摘要: BACKGROUND Disc degeneration is characterized partly by the degradation in extracellular matrix (ECM) and excess apoptosis of nucleus pulposus (NP) cells. NLRX1 (nucleotide-binding, leucine-rich repeat containing X1) different from other nucleotide-binding-domain leucine-rich-repeat proteins mainly located to mitochondrial. It negatively regulates NF-κB (nuclear factor kappa B) inhibition. However, how regulated exerts effects disc unclear. Thus, study aimed analyze on NP MATERIAL AND METHODS expression was detected interleukin (IL)-1β-induced cells western blot quantitative real-time polymerase chain reaction (qRT-PCR). Then, overexpressed IL-1β-induced detect apoptosis-related blot, along with detection levels using flow cytometry. StarBase predicted miR-423-5p target 3'UTR NLRX1. Dual luciferase reporter assay showed that could bind Besides, significantly affected qRT-qPCR. RESULTS The overexpression markedly, protective IL-1β induced our results suggested mediated regulation affect ECM CONCLUSIONS be potential therapeutic targets for patients degeneration.