Cell-free microRNA expression signatures in urine serve as novel noninvasive biomarkers for diagnosis and recurrence prediction of bladder cancer

摘要: // Lutao Du 1 , Xiumei Jiang Weili Duan Rui Wang lishui Guixi Zheng Keqiang Yan 2 Lili Juan Li Xin Zhang Hongwei Pan Yongmei Yang and Chuanxin 3 Department of Clinical Laboratory, Qilu Hospital Shandong University, Jinan, 250012, Province, China Urology, The Second Jinan 250033, Shandong, Correspondence to: Wang, email: cxwang@sdu.edu.cn Keywords: microRNA, bladder cancer, diagnosis, recurrence, urine Received: October 22, 2016     Accepted: March 14, 2017     Published: 28, 2017 ABSTRACT Urinary microRNAs (miRNAs) are potential biomarkers for the noninvasive diagnosis cancer (BC). In this study, we aimed to develop a urinary miRNAs panel diagnosing predicting recurrence BC. Genome-wide analysis by deep sequencing followed two phases quantitative real-time PCR assays were performed on supernatant 276 BC patients controls. We identified seven-miRNA (miR-7-5p, miR-22-3p, miR-29a-3p, miR-126-5p, miR-200a-3p, miR-375, miR-423-5p) that provided high diagnostic accuracy with an AUC 0.923 0.916 in training validation set, respectively. corresponding AUCs Ta, T1 T2-T4 0.864, 0.930 0.978, significantly higher than those cytology, which 0.531, 0.628 0.724, respectively (all p < 0.05). Moreover, Kaplan–Meier showed nonmuscle-invasive (NMIBC) miR-22-3p low miR-200a-3p level had worse recurrence-free survival (RFS) (p = 0.002 0.040, respectively). Multivariate Cox regression revealed independently associated RFS NMIBC 0.024 0.008, conclusion, our results suggested may have considerable clinical value prediction