Analysis of the progression of intraductal proliferative lesions in the breast by PCR-based clonal assay

摘要: Purpose To analyze the progression in patients with a morphological diagnosis of intraductal proliferative lesions by PCR-based clonal assay. Materials and methods An X-chromosome inactivation assay was applied to explore relationships human breast. Four groups samples, including 40 cases usual ductal hyperplasia (UDH), atypical (ADH), 29 flat epithelia atypia (FEA), carcinoma situ (DCIS) were selected for analysis. Thirty specimens normal breast tissue used as control group. Microdissection performed collect samples extraction genomic DNA from paraffin-embedded tissues. The subjected PCR amplification CAG repeats androgen receptor (AR) gene exon I without prior digestion methylation-sensitive restriction enzyme HhaI. Gel electrophoresis detect nature these four samples. Results analysis confirmed monoclonality all informative DCIS cells. Normal tissues majority (97.1%) UDH shown be polyclonal. Monoclonality revealed 20/39 (51.3%) ADH. Among 26 FEA, 20 polyclonal, while six displayed monoclonal alterations which accounted 23.1%. Conclusion These findings reinforce recent suggestions that AR polymerase chain reaction may define genetic among tumor lesions. Furthermore, our observations demonstrate nearly half ADH smaller part FEA have alterations, neoplastic This method would shed light on abnormalities play role early tumorigenesis breast, thus might an adjunct predicting probability occurrence guiding management cases.