Changes in CpG Islands Promoter Methylation Patterns during Ductal Breast Carcinoma Progression

摘要: Aberrant promoter methylation of several known or putative tumor suppressor genes occurs frequently during carcinogenesis, and this epigenetic change has been considered as a potential molecular marker for cancer. We examined the status nine (APC, CDH1, CTNNB1, TIMP3, ESR1, GSTP1, MGMT, THBS1, TMS1), by quantitative specific PCR. Synchronous preinvasive lesions (atypical ductal hyperplasia and/or carcinoma in situ) invasive breast from 52 patients, together with pure 24 patients 12 normal tissues paired to 20 distant were analyzed. was detected both APC, GSTP1. However, hierarchical mixed model Generalized Estimating Equations analyses showed that only CTNNB1 regions higher frequency levels pathologic samples when compared breast. Whereas APC did not show differences frequencies, CDH1 tumors (P < 0.04, Mann-Whitney test permutation correction). The analysis able distinguish between sensitivity 67% (95% confidence interval, 60-71%) specificity 75% 69-81%). Our data point direct involvement early stages cancer progression suggest they may represent useful tool detection cells clinical specimens.