Identification of a Novel Core Type in SalmonellaLipopolysaccharide COMPLETE STRUCTURAL ANALYSIS OF THE CORE REGION OF THE LIPOPOLYSACCHARIDE FROM SALMONELLA ENTERICA sv. ARIZONAE O62

摘要: Abstract For the first time, complete structure of a lipopolysaccharide (LPS) core region fromSalmonella enterica has been identified that is different from Ra type generally thought to be present in allSalmonella LPS. The LPSs two rough mutants and smooth form S. sv. Arizonae IIIa O62, which all failed react with an type-specific monoclonal antibody were resistant phage FO1, analyzed after chemical modification using monosaccharide analysis, mass spectrometry, NMR spectroscopy. In novel type, terminal d-GlcNAc residue replaced by d-Glc residue. O-specific polysaccharide α1→4-linked second distal Glc core. Furthermore, phosphoryl substituents attached O-4 ofl-glycero-d-manno-heptose (Hep) I II as 2-aminoethyl diphosphate (on Hep I) phosphate (Hep II). Abbreviations Structure are follows: Hepp,l-glycero-d-manno-heptopyranose; Kdo, 3-deoxy-d-manno-oct-2-ulopyranosonic acid; PPEA, diphosphate; R, polysaccharide. presence this LPS entericashould taken into account development general antibody-based diagnostic system forSalmonella.