Potential use of human adipose mesenchymal stromal cells for intervertebral disc regeneration: a preliminary study on biglycan-deficient murine model of chronic disc degeneration
作者： Giovanni Marfia , Rolando Campanella , Stefania Elena Navone , Ileana Zucca , Alessandro Scotti
DOI: 10.1186/S13075-014-0457-5
关键词:
摘要: Introduction: Biglycan is an important proteoglycan of the extracellular matrix intervertebral disc (IVD), and its decrease with aging has been correlated IVD degeneration. deficient (Bgn �/0 ) mice lack this protein undergo spontaneous degeneration aging, thus representing a valuable in vivo model for preliminary studies on therapies human progressive The purpose present study was to assess possible beneficial effects adipose-derived stromal cells (ADSCs) implants Bgn mouse model. Methods: To evaluate ADSC implant efficacy, were intradiscally (L1-L2) injected 8x10 4 ADSCs at 16 months old, when exhibit severe complete degeneration, evident both 7Tesla Magnetic Resonance Imaging (7TMRI) histology. Placebo treated assessed by 7TMRI analysis up 12 weeks post-transplantation. Mice then sacrificed implanted discs analyzed histology immunohistochemistry presence expression biglycan aggrecan area. Results: After treatment, revealed increase signal intensity within that received ADSCs, while placebo treatment did not show any variation. Ultrastructural analyses demonstrated survival occurred after implant. These acquired positive biglycan, specifically localized cells. Moreover, resulted significant tissue levels. Conclusion: Overall, work demonstrates into degenerated ameliorates damage, promotes new increased levels aggrecan. This suggests potential benefit chronic degenerative disease prompts further field.
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