Insulin-like growth factor I gene expression in vascular cells.

摘要: Insulin-like growth factor I (IGF I), a potent in vitro, is present blood and multiple tissues major mediator of the effects hormone on postnatal growth. IGF internalized retained largely intact cultured vascular endothelial cells. Neovasculature transiently expresses immunoreactivity, but it not known whether this represents internalization circulating or cell synthesis I. As an initial approach to defining role endogenous production program vessel wall, Northern hybridizations were performed with RNA from rat aortic smooth muscle cells bovine Rat expressed three primary messenger transcripts sized 8.2, 1.7, 0.9-1.2 kb. Bovine one minor transcript 2.1 1.6 kb, respectively. gene expression was also demonstrated by ribonuclease protection assays using exon 3 riboprobe. Both secreted I, as detected radioimmunoassay conditioned medium after separation its binding proteins gel filtration chromatography. Because stimulates cells, characterization vessels may be key understanding developmental well abnormal cardiovascular system.