Noncontact Laser Microdissection and Catapulting for Pure Sample Capture

摘要: The understanding of the molecular mechanisms cellular function, growth, and proliferation is based on accurate identification, isolation, finally characterization a specific single cell or population cells its subsets biomolecules. For simultaneous analysis thousands parameters within one experiment as realized by DNA, RNA, protein microarray technologies, defined number homogeneous derived from distinct morphological origin are required. Sample preparation therefore very crucial step preceding functional populations. Laser microdissection laser pressure catapulting (LMPC) enables pure sample resulting in an increased specificity analyses. With LMPC, force focused light utilized to excise selected large tissue areas object slides down individual subcellular components like organelles chromosomes. After microdissection, directly catapulted into appropriate collection vial. As this process works entirely without mechanical contact, it retrieval morphologically cross-contamination. LMPC has been successfully applied isolate catapult from, for example, histological sections, forensic evidence material, also tough plant matter, supporting biomedical research, science, physiology studies. Even delicate living stem have captured recultivation affecting their viability character, important feature influencing regenerative medicine, drug development. combination with microinjection inject drugs genetic material capture them analyses bears great potential efficient patient-tailored medication.