Inhibition of ornithine decarboxylase by α-difluoromethylornithine induces apoptosis of HC11 mouse mammary epithelial cells

摘要: The effect of α-difluoromethylornithine (DFMO) on the apoptosis HC11 mouse mammary epithelial cells was investigated. involvement reactive oxygen species (ROS) and Bcl-2 protein in mechanism induced by ornithine decarboxylase (ODC) inhibition also assessed. DFMO (0.1, 1 5 mM) dose- time-dependent manner. Apoptosis manifests itself with morphological features like: cell shrinkage, condensation chromatin, pyknosis fragmentation nucleus, followed secondary necrosis (putrosis). decrease nuclear DNA contents appearing as hypodiploidal peak sub-G1 histogram not dependent presence prolactin (5 μg/ml) DFMO-treated cultures. ODC associated a rapid increase ROS concentration observed within h after treatment. down-regulation number expressing bcl-2 lowered content this protooncogene noted. administration putrescine (50 μM) early-apoptotic, late-apoptotic necrotic cells. Moreover, it increased bcl-2. In conclusion, disturbance cellular polyamine homeostasis their synthesis enhances susceptibility to apoptosis. It may occur gland at end lactation, when depletion circulating lactogenic hormones activation intra-mammary apoptogenic factors expression take place.