Complete Structural Elucidation of Triacylglycerols by Tandem Sector Mass Spectrometry

摘要: We developed a method to elucidate the complete structure of triacylglycerols by means high-energy collisional activation tandem mass spectrometry (MS/MS). Both ESI- and FAB-produced [M + NH4]+ met.]+ ions (where met. = Li, Na, Cs) undergo charge-remote charge-driven fragmentations. emphasize study fragment from ESI-produced Na]+ ions. produce four types ions, NH4 - RnCOONH4]+, [RnCO 128]+, 74]+, RnCO+ which carbon number degree unsaturation each acyl group are obtained. In addition, three series produced fragmentations (CRFs), analysis their patterns gives position double bonds on groups. Information about glycerol backbone, however, is not provided collisionally activated dissociation On other hand, form eight (named A-J ions) that, like those CRF, highly structurally informative. The absence certain members also carries useful structural information. Interpretation these enables one obtain carbons, degrees unsaturation, location bonds, as well positions groups backbone.