Mycobacterium tuberculosis Rv0652 stimulates production of tumour necrosis factor and monocytes chemoattractant protein‐1 in macrophages through the Toll‐like receptor 4 pathway

摘要: Mycobacterial proteins interact with host macrophages and modulate their functions cytokine gene expression profile. The protein Rv0652 is abundant in culture filtrates of Mycobacterium tuberculosis K-strain, which belongs to the Beijing family, compared levels H37Rv CDC1551 strains. induces strong antibody responses patients active tuberculosis. We investigated pro-inflammatory production induced by murine roles signalling pathways. In RAW264.7 cells bone marrow-derived macrophages, recombinant predominantly tumour necrosis factor (TNF) monocyte chemoattractant (MCP)-1 production, was dependent on mitogen-activated kinases nuclear factor-κB. Specific pathway inhibitors revealed that extracellular signal-regulated kinase 1/2 (ERK1/2), p38 phosphatidylinositol 3-kinase (PI3K) pathways were essential for Rv0652-induced TNF whereas ERK1/2 PI3K pathways, but not pathway, critical MCP-1 macrophages. Rv0652-stimulated secretion occurred a Toll-like receptor 4-dependent MyD88-dependent manner. addition, significantly up-regulated mannose receptor, CD80, CD86 MHC class II molecules. These results suggest can induce protective immunity against M. through macrophage activation.