Intracellular Antibodies (Intrabodies) and Their Therapeutic Potential

摘要: Combining exquisite specificity and high antigen-binding affinity, intrabodies have been used as a biotechnological tool to interrupt, modulate, or define the functions of wide range target antigens at posttranslational level. An intrabody is an antibody that has designed be expressed intracellularly can directed specific antigen present in various subcellular locations including cytosol, nucleus, endoplasmic reticulum (ER), mitochondria, peroxisomes, plasma membrane trans-Golgi network (TGN) through frame fusion with intracellular trafficking/localization peptide sequences. Although different forms, most commonly format singlechain (scFv Ab) created by joining variable domains heavy light chain interchain linker (ICL), often 15 amino acid (GGGGS)(3) between (VH) (VL) chains. Intrabodies research cancer, HIV, autoimmune disease, neurodegenerative transplantation. Clinical application mainly hindered availability robust gene delivery system(s) cell delivery. This review will discuss several methods selection, strategies cellular targeting, recent successful examples applications. Taking advantage affinity for its antigen, virtually unlimited diversity available molecular techniques are emerging promising tools generate phenotypic knockouts, manipulate biological processes, obtain more thorough understanding functional genomics.