Temperature jump relaxations in aqueous saline suspensions of human erythrocytes
作者： Jeffrey D. Owen , Bruce C. Bennion , L.Paull Holmes , Edward M. Eyring , Michael W. Berg
DOI: 10.1016/0005-2736(70)90037-4
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摘要: The Joule heating temperature jump relaxation method utilizing scattered white light intensity detection has been found suitable for measuring kinetic properties of whole cells suspended in aqueous saline solutions. Human erythrocytes 0.9% NaCl and subjected to a rapid 8° 25° yield two relaxation; τ1 approx. 0.9 msec τ2 120 msec. latter appears be characteristic intact only. On the other hand, is obtained with ghosts as well erythrocytes. Similar experiments were carried out at several cell dilutions, different scattering angles, concentrations, salts, 2H2O, added sodium lauryl sulfate or 1,5-difluoro-2,4-dinitrobenzene. 0.9-nisec time constant passage water through erythrocyte membrane. principal obstacle firmer attribution unknown equilibrium interior heated solution.
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