Gene expression in Citrus sinensis (L.) Osbeck following infection with the bacterial pathogen Candidatus Liberibacter asiaticus causing Huanglongbing in Florida

摘要: Huanglongbing (HLB) (= citrus greening) is a destructive disease of which caused by fastidious, phloem-inhabiting bacterium the genus Candidatus Liberibacter. Large-scale analysis gene expression changes in 'Valencia' orange leaves were studied during course 19 weeks after inoculation with Ca. L. asiaticus using Affymetrix GeneChip 1 genome array to provide new insights into molecular basis response this pathogen. Of more than 33,000 probe sets on microarray 21,067 expressed leaves, 279 and 515 differentially (false-discovery rate (FDR) 0.05) 5-9 13-17 inoculation, respectively. Results from semi-quantitative RT-PCR performed 14 selected genes highly correlated those observed microarray. Gene involved variety different processes including cell defense, transport, cellular organization, photosynthesis, carbohydrate metabolism. Notable was pathogen-induced accumulation transcripts for phloem-specific lectin PP2-like protein. Transcriptional their relation symptom development are discussed. This first study transcriptional profiling liberibacter infection technology. Published Elsevier Ireland Ltd.