Spatial and temporal diversity of microcystins and microcystin-producing genotypes in Oneida Lake, NY
作者： Amber M. Hotto , Mike F. Satchwell , Dianna L. Berry , Christopher J. Gobler , Gregory L. Boyer
DOI: 10.1016/J.HAL.2008.02.001
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摘要: Abstract Oneida Lake is a shallow, eutrophic lake with well-established cyanobacterial population reported toxic blooms containing hepatotoxic microcystins (MC). Peak bloom events from the summers of 2002 and 2003 were analyzed to determine principal genera microcystin synthetase (mcy) genes. Sequence analysis partial mcyA amplicon targeting Microcystis, Anabaena Planktothrix sp. indicated that Microcystis was dominant mcy genotype. This clade split into two distinct sub-clades. Bloom contained members both sub-clades higher MC concentrations found when present in near equal proportions. The proportion mcyD gene ranged 0 37% total as determined by quantitative PCR (qPCR). concentration genes linearly related MCs (r2 = 0.63). relationship between genotype physiochemical variables examined factor(s) controlling periodicity production Lake. Multivariate statistical analyses, used correlate continuous-response variables, revealed strong chlorophyll a, Microcystis.
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